Drawing the Zone Line: Development of Fungal Melanin-Based Ink for Wood

Graduate Student Name: 
Hayden Houck
Email Address: 
Faculty mentor/Supervisor: 
Dr. Seri Robinson
Email Address (Faculty mentor/Supervisor): 
Department Affiliation: 
Wood Science & Engineering
Job Location: 
Richardson Hall 171
Description of project or research opportunity: 
As a member of the Robinson lab, my studies are centered around fungal decay and wood discoloration, processes which give rise to a phenomenon known as spalting. My thesis project is centered around fungal melanin, a biopolymer pigment with diverse uses and fascinating properties. I am culturing three different fungal species in various nutrient media mixtures in order to determine the optimal set of conditions for melanin production. Once the melanin is produced, I extract it from my fungal cultures and clean it up for use as the main pigment in the ink I am developing. There are many facets of this project which require a variety of laboratory skills, from sterile fungal culturing technique to melanin purification using chemistry equipment and techniques to color analysis of melanin-stained wood. The ink that is produced will ultimately be sold to woodworkers and artists who want to spalt wooden objects themselves rather than work with decayed wood. This project represents an exciting opportunity to delve into my many specialized areas of focus, including wood science and anatomy, polymer chemistry, fungal biodeterioration, pigment analysis, and more.
Tasks student will perform: 
- Prepare media for microbial cultures, safely using laminar flow hood and autoclave - Inoculate and monitor growth of fungal cultures - Collect data on mycelial growth, pigment production, and color of cultures throughout growth period - Extract and purify fungal melanin from culture media using organic chemistry lab techniques - Grind purified melanin and mull into ink
Special skills required: 
- Safe operation of: autoclave, laminar flow hood with ethanol and flame sterilization, fume hood, hot plates, and benchtop centrifuge - Safe handling of strong acids and bases, possible use of organic solvents in fume hood - Sterile technique for microbial culturing
Proposed dates of employment (must be between June 19 and September 4): 
Monday, June 23, 2025 to Thursday, September 4, 2025
Anticipated hours worked per week: 
10-15