Glyphosate (RoundUp™) is the most widely-used herbicide worldwide and while its effects on bacteria have been extensively studied, effects on fungi are less known. Understanding how long-term glyphosate use changes fungal decomposition capabilities will become increasingly important. The effect of glyphosate on wood-decay, an essential ecosystem service primarily performed by basidiomycetes, has received little attention. To explore this, we asked, “does repeated, long-term glyphosate application impact soil and wood fungal communities” and “does glyphosate exposure alter wood-decay processes?” Untreated southern pine sapwood stakes were exposed in ground contact using a matched-pair plot design (glyphosate/no glyphosate) with four blocks at each of three sites. Destructive harvests were planned every six months for five years. At each sampling (1) eight stakes/plot were visually rated for decay progression and returned, and (2) two soil samples, before and after glyphosate application, and one stake were collected for fungal community analysis of the ITS2 region using Illumina MiSeq. The sequence data will fill a knowledge gap regarding the effects of vegetation management on fungal communities. Coupling these data with a specific ecosystem function (wood-decomposition) will help gain a deeper understanding of the functional influence of targeted taxa and the impact of glyphosate on the system. This project has moved into a second phase (post 5-years) but we have a substantial amount of samples remaining from phase 1 that are in need of DNA extraction and preparation for Illumina sequencing.